DNA

Part:BBa_K315007:Design

Designed by: Anvi Raina   Group: iGEM10_Davidson-MissouriW   (2010-06-18)


Lox N forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.


Source

We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html).

References